Journal of Prevention and Treatment for Stomatological Diseases ›› 2019, Vol. 27 ›› Issue (6): 350-354.doi: 10.12016/j.issn.2096-1456.2019.06.002

• Basic Study • Previous Articles     Next Articles

Mitochondrial fission protein 1 determines mitochondrial fission and cisplatin sensitivity in tongue squamous cell carcinoma

Xinyuan LEI,Xinyu LIN,Zhanpeng OU,Yi RUAN(),Jinsong LI()   

  1. Department of Stomatology, Sun Yat-sen Memorial hospital, Sun Yat-sen University,Guangzhou 510120, China
  • Received:2019-03-07 Revised:2019-04-02 Online:2019-06-20 Published:2019-06-21
  • Contact: Yi RUAN,Jinsong LI E-mail:13802929651@139.com;lijinsong1967@163.com

Abstract:

Objective To investigate the effect of mitochondrial fission protein 1 (FIS1) on apoptosis and cisplatin resistance in tongue squamous cell carcinoma (TSCC) cells. Methods The squamous cell carcinoma cell lines SCC9 and CAL27 were used to detect the mRNA and protein levels of FIS1 after cisplatin treatment, the knockdown and overexpression of FIS1 of SCC9 and CAL27 with or without cisplatin treatment were accomplished through small interfering RNA (siRNA) and plasmid, respectively. The mitochondrial division state in cells was detected by mitochondrial staining, and the apoptosis state of cells was detected by TUNEL, flow cytometry and Caspase 3/7. Results FIS1 protein expression in tongue squamous carcinoma cells treated with cisplatin was increased, but the mRNA level did not change. Silencing of FIS1 expression reduced mitochondrial division and apoptosis in squamous cell carcinoma cells treated with cisplatin, whereas overexpression of FIS1 exhibited the opposite effects. The percentage of dividing mitochondria, the number of apoptotic cells and the activity of Caspase 3/7 in SCC9 and CAL27 cells were significantly different before and after modulation of FIS1 expression (P < 0.05). Conclusion FIS1 is involved in the regulation of cisplatin chemotherapy sensitivity in tongue squamous cell carcinoma and can be used as a new target for improving the sensitivity of cisplatin chemotherapy in oral squamous cell carcinoma.

Key words: Mitochondrial dynamics, Tongue squamous cell carcinoma, Cisplatin sensitivity, FIS1, Chemotherapy, Apoptosis, Small interfere RNA

CLC Number: 

  • R739.8

Figure 1

Expression of FIS1 and mitochondrial fission in tongue squamous cell carcinoma cells treated with cisplatin "

Figure 2

Silencing FIS1 reversed cisplatin-induced mitochondrial fission and apoptosis "

Figure 3

Overexpression of FIS1 promoted mitochondrial fission and apoptosis"

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