Journal of Prevention and Treatment for Stomatological Diseases ›› 2016, Vol. 24 ›› Issue (7): 390-394.DOI: 10.12016/j.issn.2096-1456.2016.07.003

• Basic Study • Previous Articles     Next Articles

Effects of LMK-235 on osteoblast/odontoblast differentiation in hPDLCs

Qian-qian HAN1,Zhao LIU2,Li JIANG1,Hui-yi TANG1,Xiao-na LI3()   

  1. 1. Department of Periodontology, Guangdong Provincial Stomatological Hospital, the Affiated Stomstological Hospital of Southern Medical University, Guangzhou 510280, China
  • Received:2016-03-12 Revised:2016-04-07 Online:2016-07-20 Published:2016-07-20
  • Contact: Xiao-na LI

LMK-235对牙周膜细胞成骨和成牙本质分化的影响

韩倩倩1,刘钊2,江丽1,汤慧怡1,李晓娜3()   

  1. 1. 广东省口腔医院·南方医科大学附属口腔医院牙周病科, 广东 广州(510280)
    2. 南方医科大学南方医院牙体牙髓病科
    3. 广东省口腔医院·南方医科大学附属口腔医院牙体牙髓病科
  • 通讯作者: 李晓娜
  • 作者简介:韩倩倩,住院医师,博士, Email: hanqianqian04@163.com
  • 基金资助:
    国家自然科学基金青年项目(81500848);广东省自然科学基金博士启动项目(2014A030310405);广东省医学科研基金(A2015196,A2015200)

Abstract:

Objective To investigate the effects of type Ⅱa histone deacetylase inhibitor LMK-235 during early osteoblast/odontoblast differentiation in hPDLCs.Methods hPDLCs were obtained by the collagenase digestion method. hPDLCs at the 3 rd passage were treated with medium containing 10% fetal bovine serum mixed with different concentrations of LMK-235 (0, 50, 100, 250, 500 nmol/L), respectively. Proliferative capability of hPDLCs was tested by MTT and qRT-PCR was used to detect mRNA expression levels of Runx2, ALP and DMP-1 3 d later. Results MTT assay showed that cell proliferation in hPDLCs treated with 100 nmol/L LMK-235 was increased significantly compared with the control group (P<0.05). The expression of Runx2 mRNA in the 100 nmol/L group was 1.77 times of the control groups (P<0.05). The expressions of ALP mRNA in all the experimental groups were significantly higher than that in control groups (P<0.05), and the expression in the 100 nmol/L groups was the highest. The expressions of DMP-1 mRNA in the 50 and 100 nmol/L groups were higher than the control groups (P<0.05). Conclusion Type Ⅱa histone deacetylase inhibitor LMK-235 could accelerate cell proliferation in hPDLCs at the concentration of 100 nmol/L, and regulate early osteoblast/odontoblast differentiation by upregulating the mRNA expressions of Runx2, ALP and DMP-1.

Key words: Periodontal ligament cells, Osteoblastic differentiation, Odontoblast differentiation, Histone deacetylase, Cell proliferation

摘要:

目的 探讨Ⅱa类组蛋白去乙酰化酶抑制剂LMK-235对人牙周膜细胞(human periodontal ligament cells, hPDLCs)早期成骨和成牙本质分化的影响。方法 通过酶消化法获得hPDLCs,分别用浓度为0、50、100、250、500 nmol/L的LMK-235处理第三代hPDLCs 3 d。MTT法检测hPDLCs的增殖,同时qRT-PCR检测成骨及成牙本质相关因子Runx2、ALP 及DMP-1 mRNA的表达水平。结果 MTT结果显示100 nmol/L 的LMK-235对hPDLCs增殖具有促进作用。qRT-PCR结果表明100 nmol/L处理组Runx2 mRNA的表达水平为对照组的1.77倍(P<0.05);而ALP mRNA的表达水平在实验组均高于对照组(P<0.05),同时100 nmol/L处理组表达量最高;DMP-1 mRNA的表达水平在50 nmol/L及100 nmol/L组较对照组升高(P<0.05)。结论 浓度为100 nmol/L的Ⅱa类组蛋白去乙酰化酶抑制剂LMK-235促进hPDLCs增殖,并通过上调Runx2、ALP、DMP-1等成骨及成牙本质相关因子mRNA表达来促进hPDLCs早期成骨及成牙本质分化。

关键词: 牙周膜细胞, 成骨分化, 成牙本质分化, 组蛋白去乙酰化酶, 细胞增殖

CLC Number: