Journal of Prevention and Treatment for Stomatological Diseases ›› 2016, Vol. 24 ›› Issue (11): 640-644.doi: 10.12016/j.issn.2096-1456.2016.11.004

• Basic Study • Previous Articles     Next Articles

Effects of exogenous dextranase and sodium fluoride on Actinomyces viscosus biofilm

TIAN Yuan-yuan1,2,YU Xuan1,3,FANG Hong-zhi2,YANG Ying-ming1,4,YANG Hui1,5(),HU Tao1,4   

  1. 1. State Key Laboratory of Oral Diseases, Department of Endodontics, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China
    2. Department of Stomatology, The Third People's Hospital of Chengdu, Chengdu 610041, China
    3. Department of Stomatology, Yinzhou Hospital Affiliated to Medical School of Ningbo University, Ningbo 315040, China
    4. Department of Preventive Dentistry, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China
    5. Department of General Dentistry, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China
  • Received:2016-07-01 Revised:2016-07-26 Online:2016-11-20 Published:2016-11-20
  • Contact: Hui YANG

Abstract: Objective To explore the impact of exogenous dextranase (Dex) and sodium fluoride (NaF) on the biofilm formation of Actinomyces viscosus. Methods Crystal violet was used to quantify the biomass of Actinomyces viscosus treated with serial concentrations of Dex and NaF. Scanning electronic microscope was applied to detect the morphology and structure of the biofilms. Results The formation of Actinomyces viscosus biofilm was inhibited when the concentrations of Dex or NaF rised. Compared with the separate use of 0.25 U/mL Dex and 40 or 80 μg/mL NaF, the combined use of these two agents could obviously inhibit the biofilm formation. Conclusion Both separate and combined use of Dex and NaF could inhibit the Actinomyces viscosus biofilm formation, and they displayed synergistic effect under certain conditions.

Key words: Dextranase, Sodium fluoride, Actinomyces viscosus, Biofilm, Synergistic effect

CLC Number: 

  • R781.1

Figure 1

The biofilms morphology of Actinomyces viscosus in different groups detected by SEM"

Table 1

The biomass of Actinomyces viscosus in different experimental groups"

实验分组 吸光度 (OD590) 值 P
空白对照组 0.946±0.059
20 μg/mL NaF组 0.902±0.021 0.125
40 μg/mL NaF组 0.677±0.058 0.000
80 μg/mL NaF组 0.691±0.057 0.000
160 μg/mL NaF组 0.678±0.038 0.000
320 μg/mL NaF组 0.524±0.022 0.000
0.1 U/mL Dex组 0.909±0.031 0.162
0.25 U/mL Dex组 0.667±0.033 0.000
0.5 U/mL Dex组 0.213±0.032 0.000
1 U/mL Dex组 0.079±0.003 0.000
0.25 U/mL Dex + 40 μg/mL NaF组 0.217±0.029 0.000
0.25 U/mL Dex + 80 μg/mL NaF组 0.304±0.059 0.000
0.5 U/mL Dex + 40 μg/mL NaF组 0.157±0.012 0.000
0.5 U/mL Dex + 80 μg/mL NaF组 0.126±0.017 0.000
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