Research on the inhibitory effect of Baicalin on the human tongue squamous cell carcinoma cell line SCC15

doi:10.12016/j.issn.2096-1456.2019.04.004

Abstract

Abstract:

Objective To explore the inhibitory effect and possible mechanism of Baicalin on the human tongue squamous cell carcinoma cell line SCC15 and to provide a new idea and experimental basis for the clinical prevention and treatment of tongue squamous cell carcinoma.Methods SCC15 cells cultured in DMEM alone were used as the control group, and SCC15 cells cultured in 20 mg/mL baicalin solution were used as the baicalin group. Scratch tests and Transwell migration tests were performed to detect changes in cell migration ability, and flow cytometry was used to detect changes in the cell cycle. Western blotting was used to detect differences in the phosphorylation levels of signal transduction and transcription activator 3 (STAT3).Results Compared with the control group, the scratch test and the Transwell migration test showed that the cell migration ability of cells in the baicalin group was significantly decreased (t=4.927, P=0.008); flow cytometry showed that the number of cells of the baicalin group increased in the G0/G1 phase (t=9.893, P=0.001), decreased in the S phase (t=8.528, P=0.001), and decreased in the G2/M phase (t=3.550, P=0.024); Western blotting results showed that the STAT3 protein of SCC15 cells in the baicalin group decreased (t=3.550, P=0.024), and the phosphorylation level significantly decreased (t=8.262, P=0.001).Conclusion Baicalin inhibits the human tongue squamous cell carcinoma cell line SCC15, and its mechanism may be related to a decrease in STAT3 pathway phosphorylation activity.

Key words: Baicalin, Tongue, Carcinoma, Squamous cell, SCC15 cell, Migration, Cell cycle, Signal transduction and transcription activator 3(STAT3)

摘要：

目的探讨黄芩苷对人舌鳞癌细胞株SCC15细胞的抑制作用及可能机制,为舌鳞癌的防治提供新的思路及实验基础。方法单纯DMEM培养液培养的SCC15细胞为对照组,加入20 mg/mL黄芩苷溶液培养的SCC15细胞为黄芩苷组。2组细胞分别以划痕实验及Transwell迁移实验检测细胞迁移能力的改变,流式细胞术检测细胞周期的改变,Western blotting检测细胞信号转导与转录激活子3（signal transduction and transcription activator 3,STAT3）磷酸化水平的差异。结果与对照组比较,划痕实验及Transwell迁移实验结果显示黄芩苷组细胞迁移能力降低（t=4.927,P=0.008）;流式细胞术结果显示黄芩苷组G0/G1期增高（t=9.893,P=0.001）,S期降低（t=8.528,P=0.001）,G2/M期降低（t=3.550,P=0.024）;Western blotting结果显示黄芩苷组SCC15细胞STAT3蛋白磷酸化水平下降（t=8.262,P=0.001）。结论黄芩苷对人舌鳞癌细胞SCC15具有抑制作用,其机制可能与降低STAT3通路磷酸化活性有关。

关键词: 黄芩苷, 舌, 癌, 鳞状细胞, SCC15细胞, 迁移, 细胞周期, 信号转导与转录激活子3

CLC Number:

R739.8

ZHONG Wende,LIU Rongjing,GUAN Hongbing,CHEN Guangsheng. Research on the inhibitory effect of Baicalin on the human tongue squamous cell carcinoma cell line SCC15[J]. Journal of Prevention and Treatment for Stomatological Diseases, 2019, 27(4): 226-230.

钟文德,刘荣静,管红兵,陈广盛. 黄芩苷对人舌鳞癌细胞SCC15的抑制作用[J]. 口腔疾病防治, 2019, 27(4): 226-230.

Figures/Tables 4

Figure 1 The influence of baicalin on SCC15 cell migration

Figure 2 Flow cytometry results

Table 1 The influence of baicalin on the cell cycle of SCC15 cells $\bar{x}$ ± s

分组	G0/G1期	S期	G2/M期
对照组	64.21 ± 1.82	20.07 ± 1.13	15.72 ± 2.30
黄芩苷组	77.75 ± 1.52	11.42 ± 1.35	10.83 ± 0.63
t值	9.893	8.528	3.550
P值	0.001	0.001	0.024

Figure 3 The effect of baicalin on the phosphorylation activity of STAT3 in SCC15 cells

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