Journal of Prevention and Treatment for Stomatological Diseases ›› 2019, Vol. 27 ›› Issue (6): 355-359.doi: 10.12016/j.issn.2096-1456.2019.06.003

• Basic Study • Previous Articles     Next Articles

The role of APOD in the proliferation and migration of human dental pulp cells

XU Shuaimei,ZENG Xiongqun,YUAN Peiyan,LIU Zhongjun(),ZENG Shuguang()   

  1. Departments of Endodontics, Stomatological Hospital, Southern Medical University, Guangzhou 510280, China.
  • Received:2018-11-05 Revised:2019-01-06 Online:2019-06-20 Published:2019-06-21
  • Contact: Zhongjun LIU,Shuguang ZENG E-mail:153093518@qq.com;sunrisezdoctor@163.com

Abstract:

Objective To explore the role of apoliprotein D (APOD) in the proliferation and migration of human dental pulp cells (DPCs) and to provide a basis for the use of APOD to promote pulp regeneration. Methods APOD expression in human dental pulp cells was inhibited by siRNA. The inhibition effect of APOD was confirmed by qPCR and Western blot. After APOD inhibition, colony formation experiments and CCK8 assays were employed to confirm the proliferation ability of dental pulp cells. Transwell assays were used to verify the cell migration ability after the inhibition of APOD expression. Results After inhibiting APOD expression, the colony formation rate in the si-apod group was reduced compared with the NC group, and the difference was statistically significant (t=7.624, P=0.002). The CCK8 experiment showed that the OD value in the si-apod group decreased at 3, 5 and 7 d compared with that in the NC group (P < 0.05). Transwell results showed that the number of cell divisions was 57.25 ± 4.03 in the si-apod group and 154.50 ± 8.39 in the NC group, and the difference was statistically significant (t=10.45, P < 0.001). Conclusion Inhibition of APOD expression in dental pulp cells inhibits their proliferation and migration ability.

Key words: Apolipoprotein D, Pulp tissue regeneration, Dental pulp cells, Proliferation, Migration, CCK8

CLC Number: 

  • R781.3

Figure 1

Dental pulp cells were derived from dental pulp tissue explants × 100"

Figure 2

RNA and protein expression levels following transfection with siRNA in dental pulp cells"

Figure 3

Colony formation experiment"

Figure 4

CCK8 assay for detecting the proliferative capacity of dental pulp cells"

Figure 5

Transwell assay for detecting migration of dental pulp cells "

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