Abstract:

Objective To explore the role of apoliprotein D (APOD) in the proliferation and migration of human dental pulp cells (DPCs) and to provide a basis for the use of APOD to promote pulp regeneration. Methods APOD expression in human dental pulp cells was inhibited by siRNA. The inhibition effect of APOD was confirmed by qPCR and Western blot. After APOD inhibition, colony formation experiments and CCK8 assays were employed to confirm the proliferation ability of dental pulp cells. Transwell assays were used to verify the cell migration ability after the inhibition of APOD expression. Results After inhibiting APOD expression, the colony formation rate in the si-apod group was reduced compared with the NC group, and the difference was statistically significant (t=7.624, P=0.002). The CCK8 experiment showed that the OD value in the si-apod group decreased at 3, 5 and 7 d compared with that in the NC group (P < 0.05). Transwell results showed that the number of cell divisions was 57.25 ± 4.03 in the si-apod group and 154.50 ± 8.39 in the NC group, and the difference was statistically significant (t=10.45, P < 0.001). Conclusion Inhibition of APOD expression in dental pulp cells inhibits their proliferation and migration ability.

Key words: Apolipoprotein D, Pulp tissue regeneration, Dental pulp cells, Proliferation, Migration, CCK8

摘要：

目的 探讨载脂蛋白D（apoliprotein D,APOD）在人牙髓细胞增殖及迁移过程中的作用,为运用APOD促进牙髓组织再生提供基础。方法 设置阴性对照（NC）组及抑制APOD表达（si-APOD）组,以siRNA抑制APOD在人牙髓细胞的表达, qPCR与Western blot检测APOD表达水平;通过细胞克隆形成实验、CCK8实验检测抑制APOD表达后牙髓细胞的增殖能力;Transwell实验检测抑制APOD表达后牙髓细胞迁移能力的变化。结果 抑制APOD表达后,si-APOD组较NC组克隆形成数量降低,差异有统计学意义（t=7.624, P=0.002）; CCK8实验显示si-APOD组OD值在3、5、7 d时间点与NC组相比均降低（P < 0.05）;Transwell结果表明,si-APOD组细胞穿孔数量57.25 ± 4.03,NC组细胞穿孔数量154.50 ± 8.39,差异有统计学意义（t=10.45, P < 0.001）。结论 抑制牙髓细胞APOD的表达可抑制其增殖与迁移能力。

关键词: 载脂蛋白D, 牙髓组织再生, 牙髓细胞, 增殖, 迁移, CCK8