Journal of Prevention and Treatment for Stomatological Diseases ›› 2019, Vol. 27 ›› Issue (6): 360-364.doi: 10.12016/j.issn.2096-1456.2019.06.004

• Basic Study • Previous Articles     Next Articles

Expression of NF-κB in oral squamous cell carcinoma and paracancerous tissues and its significance

GUI Xinwei,YAO Xiaowu(),CHEN Shisheng,LU Zizheng,LIN Minxiao,PENG Yu   

  1. Department of Stomatology, The Second Affiliated Hospital , Medical College of Shantou University,Shantou 515041,China
  • Received:2019-01-01 Revised:2019-03-10 Online:2019-06-20 Published:2019-06-21
  • Contact: Xiaowu YAO E-mail:stumcdenty@163.com

Abstract:

Objective To investigate the expression and significance of NF-κB in oral squamous cell carcinoma and paracancerous tissues. Methods Cancerous and paracancerous tissues about 5 mm outside the tumor boundary were cut from 47 patients with OSCC. NF-κB expression in these tissues were detected by the immunohistochemical SP method and compared with that in 32 normal oral mucosa tissues. Results The average optical density (OD value) of immunohistochemical-positive cells in cancer tissues and paracancerous tissues was higher than that in normal tissues, and the difference was statistically significant (P < 0.001). There was no significant difference in the OD values between cancer tissues and paracancerous tissues (P=0.356). The rate of NF-κB positive expression in cancer tissue and paracancerous tissue was significantly higher than that in normal mucosa tissue, and the difference was statistically significant (P < 0.001). There was no significant difference in the rate of NF-κB positive expression between cancer tissues and paracancerous tissues (P=0.117), but the rate of moderate-to-strong positive expression in cancer tissues was significantly higher than that in paracancerous tissues (P < 0.001). Conclusion NF-κB may be involved in the development of OSCC.

Key words: Oral neoplasms, Carcinoma, squamous cell, paracancerous tissues, NF-κB;

CLC Number: 

  • R739.8

Figure 1

HE results of three kinds of tissues"

Figure 2

Immunohistochemistry results of three kinds of tissues"

Table 1

OD value of immunohistochemical-positive cells in three kinds of tissues x ± s"

组别 例数(n) OD值
癌组织 47 0.292 6 ± 0.076 61)
癌旁组织 47 0.275 3 ± 0.116 31)
正常黏膜组织 32 0.105 9 ± 0.067 6

Table 2

Immunohistochemical grading results in three kinds of tissues"

评分等级 正常黏膜组织 癌旁组织 癌组织
23 4 0
+ 9 9 0
++ 0 16 24
+++ 0 18 23
合计 32 47 47
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