Journal of Prevention and Treatment for Stomatological Diseases ›› 2019, Vol. 27 ›› Issue (8): 490-495.doi: 10.12016/j.issn.2096-1456.2019.08.003

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Effect of platelet-rich fibrin extract on the proliferation of gingival fibroblasts

HE Jialin1,2,XU Yan1(),XIE Xianzhe1,2,WANG Tengfei1,HUO Dongmei1   

  1. 1. Department of Periodontology, Affiliated Stomatology Hospital of Anhui Medical University, Hefei 230032, China
    2. Anke Bioengineering Limited Company, Hefei 230032, China
  • Received:2018-12-27 Revised:2019-04-13 Online:2019-08-20 Published:2019-08-16
  • Contact: Yan XU E-mail:173236344@qq.com

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Abstract:

Objective To study the effects of platelet-rich fibrin extract (PRFe) and platelet-derived growth factor (PDGF) released from PRFe on the proliferation of human gingival fibroblasts (HGFs) and to provide an experimental basis for its application in promoting gingival soft tissue increment.Methods Platelet-rich fibrin (PRF) was transformed into PRFe by tissue culture. The three-dimensional structure of PRF was observed by electron microscopy, and the content of PDGF in PRF was quantitatively determined by ELISA. The ratios of PRFe examined were 2.5% PRFe, 5% PRFe, 7.5% PRFe, 10% PRFe, 12.5% PRFe and 15% PRFe. Gingival fibrosis was detected by the CCK-8 method. After determining the optimal concentration of PRFe, flow cytometry was used to detect the effect of PRFe on the proliferation cycle of human gingival fibroblasts, and the effect of PDGF on the proliferative activity of gingival fibroblasts was observed by neutralizing the release of PDGF.Results PRF is a three-dimensional reticular structure that contains a large number of growth factors. PDGF release peaked on the 7th day. The proliferative activity of HGFs cultured with different concentrations of PRFe was concentration-dependent, but the effect was optimal at 5% PRFe (P < 0.05). There were no significant differences in the effect of subsequent concentration increases on the proliferation of HGFs (P > 0.05). The flow cytometry results showed that 5% PRFe could significantly stimulate the S-phase division and proliferation of gingival fibroblasts, while the PDGF neutralization test showed that the proliferation of gingival fibroblasts was significantly inhibited by the neutralization of PDGF.Conclusion Overall 5% PRFe had the best effect on promoting gingival fibroblast proliferation in vitro. PDGF released from PRF plays an important role in promoting the proliferation of gingival fibroblasts.

Key words: platelet-rich Fibrin extract, PDGF growth factor, gingival fibroblasts, cell proliferation, gums, soft tissue increment

CLC Number: 

  • R783.5

Figure 1

Gingival fibroblasts × 200"

Figure 2

Identification of human gingival fibroblasts by anti-vimentin staining and anti-cytokeratin immunohistochemical staining × 400"

Figure 3

Scanning electron microscopy of PRF"

Figure 4

Quantitative detection of platelet-rich fibrin release by PDGF ELISA"

Table 1

Comparison of mean absorbance values in each group at different time points $\bar{x}$ ± s"

时间 对照组 2.5%PRFe 5%PRFe 7.5%PRFe 10%PRFe 12.5%PRFe 15%PRFe F值 P值
1 d 0.417 ± 0.023 0.537 ± 0.008 0.574 ± 0.024 0.614 ± 0.0251） 0.658 ± 0.0691） 0.647 ± 0.011） 0.684 ± 0.0141） 7.346 0.030
3 d 0.683 ± 0.022 1.301 ± 0.1711） 1.461 ± 0.0371） 1.457 ± 0.0751） 1.41 ± 0.1071） 1.478 ± 0.0921）2) 1.502 ± 0.061)2） 52.553 0.028
5 d 0.932 ± 0.041 1.627 ± 0.0511） 2.011 ± 0.0241）2） 2.004 ± 0.0241）2） 2.011 ± 0.0311）2） 1.931 ± 0.0191）2） 1.966 ± 0.0481）2） 226.428 0.011

Figure 5

Effect of anti-PDGF on gingival fibroblast proliferation"

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