Dental operating microscope is recommended to complicated root canal therapy, for it could provide increased lighting and superior magnification, so that the survival rate of tooth is highly increased. More refined access of cavity preparation, canal cleaning, shaping and obturating are realized under microscope. This article combined literature reviews with the writers’ clinical experience, and it aims at helping clinicians to improve their ability to more accurately performance and minimally invasive endodontic procedures, such as locating hidden canals obstructed by calcifications, canals reduced in size, removing materials, reducing ledge formation, apical transportation, and repairing perforations.
Objective To investigate the morphology and proliferation viability in oxidative stress induced damage in human MG63 cells. Methods The MG63 cells were treated with superoxide anion (O2.') produced by different concentrations of xanthine/xanthine oxidase enzymatic reactions to establish the model of oxidative stress in MG63 cells, using the xanthine oxidase inhibitor oxypurinol to observe the reverse effect of oxypurinol on xanthine/xanthine oxidase induced damage in human MG63 cells. Using the flow cytometry, the production of intracellular reactive oxygen species (ROS) induced by xanthine/xanthine oxidase induced cellular oxidative stress damage was evaluated by the oxidation?sensitive fluorescent probe, the 2’7' dichlorofluorescin diacetate. Cellular viability and morphology was evaluated by the MTT assay and the phase contrast microscope. Results Xanthine/xanthine oxidase induced intracellular ROS production in a dose and time dependent manner (P < 0.05). The cellular viability was reduced and cellular morphology was damaged, too (P < 0.05). Xanthine/xanthine oxidase induced the damage of the cellular morphology. At the same processing time, the higher the xanthine/xanthine oxidase concentration, the higher intracellular ROS fluorescence intensity value, and the lower OD value, the difference was statistically significant (P < 0.05). The intracellular mean ROS fluorescence intensity in xanthine/xanthine oxidase + oxypurinol combined treatment group was significantly lower compared with the same concentration of xanthine/xanthine oxidase (P < 0.05). At the same concentration of xanthine/xanthine oxidase, with the extension of treatment time, the intracellular mean ROS fluorescence intensity gradually increased, the OD value decreased, compared with the control group, the intracellular mean ROS fluorescence intensity of 120 min increased to 345% of the control, was the highest among the xanthine/xanthine oxidase groups. The OD value of 24 h was the 22.9% of the control group, was the lowest among the xanthine/xanthine oxidase groups, cell proliferation activity decreased more obvious. Conclusions Xanthine/xanthine oxidase could induce oxidative stress damaged the cellular morphology and reduced the cellular viability in MG63 cell lines. The oxypurinol (the inhibitor of xanthine oxidas) could reverse the oxidative stress injury induced by xanthine/xanthine oxidase in human osteoblastic cells.
Objective To evaluate the relationship between-590C/T polymorphisms of interleukin-4 gene and periodontitis by meta-analysis. Methods Databases including PubMed, EMbase, Web of Science, CBM, CNKI, Wanfang and VIP, were searched from their establishment to December 31, 2016. Only case-control studies on the association between -590C/ T polymorphisms of interleukin-4 gene and periodontitis were selected, and the references of those selected studies were searched by hand. Assessment and data extraction were conducted by 2 reviewers independently, and the meta-analysis was performed with the R 3.3.1 software.Results The meta-analysis showed that there was no association between the IL-4-590C/T polymorphism and periodontitis susceptibility (T vs C: OR=1.19, 95%CI=0.81~1.76, P =0.37). Subgroup analysis showed that the IL-4 -590 TT was associated with periodontitis in Caucasians (TT vs CT: OR=1.75, 95%CI=1.10 ~ 2.78, P = 0.02), but no significant association in Asians (P =0.63). Meanwhile, IL-4 gene polymorphisms were not significantly associated with the susceptibility of chronic periodontitis (CP) or aggressive periodontitis (AgP) ( e. g. T vs C, CP: P=0.45; AgP: P =0.45). Conclusion Based on current evidence, the polymorphisms of IL-4-590C/T are associated with the increased risk of periodontitis in Caucasians.
Objective To investigate the changes of vaspin and TNF-α levels in gingival crevicular fluid (GCF) of type 2 diabetic (T2DM) patients with chronic periodontitis (CP) after non-surgical periodontal treatment. Methods 60 subjects were divided into 4 groups: DM-CP group (patients with both T2DM and CP, n=15); CP group (CP patients without T2DM, n=15); DM group (T2DM patients without CP, n=15), and CTRL group (systemically and periodontally healthy individuals, n=15). The clinical parameters of periodontal tissue and GCF were measured before and 8 weeks after non-surgical periodontal treatment. Results The levels of vaspin and TNF-α were measured by ELISA. The levels of vaspin and TNF-α in CP group were significantly higher than those in CTRL group (P < 0.05), while the levels of vaspin and TNF-α in CP group were significantly decreased after treatment (P < 0.05). There was a statistically significant positive correlation between the total amount of vaspin and the total amount of TNF-α, the level of HbA1c, gingival index (GI) and probing depth (PD) (P < 0.05). Conclusion The results shows that vaspin and TNF-α are greatly decreased in periodontitis after non-surgical periodontal treatment. It suggests that vaspin and TNF-α in GCF may serve as inflammatory markers for the diagnosis and prognosis of diabetes and periodontitis.
Objective This study aims to evaluate the shaping capability of Pathfile and WaveOne in simulated root canals. Methods A total of 20 simulated resin blocks were divided randomly into two groups, group W prepared with WaveOne alone. Group PW prepared with WaveOne and Pathfile. Preparation time and number of separated instruments were measured respectively. Pre-and post-operative images were obtained with a scanner and superimposed through Photoshop and ImageJ2X. The changes in canal bending curvature and material removal from the inner and outer canal walls at 10 points beginning at 1mm from the end point of the canal were measured with ImageJ2X. Centering capability was determined accordingly by comparing and analyzing the material removal from the inner and outer canal walls.Results Two groups have no occurrence of instrument separation, no statics significance in root canal preparation and the changes of root canal working length differences (P > 0.05). But at position of root canal point 4 to 5 mm, the misalignment of group W were 0.085 ± 0.05 mm and -0.312 ± 0.068 mm, the misalignment of group PW were -0.071 ± 0.042 mm, -0.26 ± 0.103 mm (P < 0.05).Conclusion Both two groups instruments showed good performance in preparation but both created some apical transportation. However, group PW exhibited more superior centering capability than group W in the middle part of the canal.
Objective To investigate the morphology and position changes of displaced disk with reduction after treatment by modified Twin-block. Methods 19 patients were diagnosed as displaced disk with reduction and they were treated with modified Twin-block from July 2015 to June 2016. 28 temporomandibular joints (TMJ) of these patients were included in the analysis. The disk length, disk-condylar distance, and disk-condylar angle were measured with MRI before and after treatment. Paired t-test was used. Disk morphology before and after treatment was also documented and analyzed by Wilcoxon signed rank test. The statistical significance was set at P<0.01.Results 28 TMJ disks were anteriorly displaced with reduction. 24 of them were repositioned while the other 4 were still anteriorly positioned after treatment. The disk length was increased significantly (P<0.01) while the disk-condylar distance and disk-condylar angle were decreased significantly after treatment (P<0.01). The disk morphology as hemiconvex (16) and bi planar (9) were the majority before treatment, while biconcave (16) and biplanar (10) were changed to the larger part after treatment. There was improvement on the disk deformation with a statistical significance (P<0.01). Larger disk-condylar distance, disk-condylar angle and severer deformation of disks were observed in the 4 disks without reposition.Conclusion Modified twin-block is an effective appliance for disk displacement with reduction by repositioning the disk and modifying the disk deformation. However the effect is not good for disks with severer deformation and displacement. Further studies with larger sample and stratified group are still needed.
Objective To explore the clinical effect of personalized titanium mesh combined with free flap in the repair of maxillary defect.Methods 36 cases of maxillary defect patients as the research object were selected in our hospital during May of 2010 to May of 2016. 36 defect cases were repaired with personalized titanium mesh combined with free flap, and summarize the treatment programs to explore the value of clinical application.Results By the end of follow-up, all of the flap survived, tumor recurrence rate was 5.56% with paitient satisfaction was 100%; Diplopia and dysphagia occurred in no cases; Masticatory function declined accompanied with a longer chewing time but language communication was not affected.Conclusion The application of personalized titanium mesh and free flap repair methods in maxillary defect is significant, which effectively improve the quality of life of patients whereas still have difficulties in late denture at the same time.
Objective To investigate the feasibility of using UV curable calcium hydroxide as temporary crown binder. Methods 242 patients with temporary crown were selected and randomly divided into three groups according the order of registration week each month, and respectively underwent temporary crown cement with TempoCemNE (n = 78), Provicol (n = 80) and Calcimol LC (n = 84) for each tooth. The retention effect and the status of the gum and the pulp were observed at one week after operation.Results There was no significant difference between the three groups in the retention effect and the status of the gum and the pulp (χ12 = 5.553, P1 = 0.062 > 0.05; χ22 = 4.783, P2 = 0.091 > 0.05; χ32 = 3.290, P3 = 0.193 > 0.05). There was a good effect of temporary crown cement in three groups of TempoCemNE, Provicol and Calcimol LC.Conclusion Calcimol LC can be used as a new type of temporary crown cement with good bonding performance and the protection to gums and pulp, and simple operation.
Objective To compare the depth of the curve of Spee in Angle classⅡ malocclusion patients with different vertical skeletal patterns and to investigate the relationship between the depth of the curve of Spee and dentofacial morphology. Methods 101 Angle classⅡ malocclusion patients were selected and randomly divided into 3 groups based on their GoGn-SN angles-high angle, average angle and low angle. Lateral cephalograms and dental models of all patients were evaluated to analyze Spee curve depth difference among different groups, Correlation analysis and a multiple linear regression analysis were performed to determine the relationship between the depth of the curve of Spee and all selected cephalometric variables.Results There was statistical difference in depth of the curve of Spee among different groups P < 0.05. The depth of the curve of Spee was least in the high angle group and greatest in the low angle group. GoGn-SN angle had statistically significant negative correlation with the depth of the curve of Spee, r = 0.428, P = 0.000, ODI, S-Go/N-Me、L7-GoGn angle had statistically significant positive correlation with the depth of the curve of Spee, r = 0.381, 0.357, 0.333, P = 0.000, 0.000, 0.001. The multiple linear regression analysis with stepwise method showed GoGn-SN angle had significant contribution to the depth of the curve of Spee. In Angle classⅡ malocclusion patients, there was statistical difference in depth of the curve of Spee among different vertical skeletal patterns.Conclusion The depth of the curve of Spee is correlated with dentofacial morphology, GoGn-SN angle had significant contribution to the depth of the curve of Spee, which should be taken into consideration during orthodontic diagnosis and treatment.
Objective To study the effect of orthodontic treatment on alveolar bone density and epidermal growth factor (EGF) expression in patients with periodontal disease. Methods 50 patients with periodontal disease admitted into our hospital from January 2015 to January 2016 were selected as research object. Results Before treatment, patients were given dental health instruction. Under the conditions of patients agreeing and cooperating, patients received orthodontic treatment. The changes of alveolar bone density, EGF in blood, saliva, gingival crevicular fluid (GCF), indexes of periodontal inflammation (gingival index, papilla Bleeding index, probing depth) of patients before and after treatment for one month were compared. Before treatment, the mandibular alveolar bone density was significantly higher than maxillary alveolar bone density (P < 0.05); After treatment, the maxillary and mandibular alveolar bone density were significantly lower than before treatment, and maxillary bone density was significantly lower than mandibular bone density (P < 0.05); After one-month treatment, the concentration of EGF expression in blood, saliva compared with before treatment, there was no statistical significance (P > 0.05); After one-month treatment, the concentration of EGF expression in GCF was significantly higher than before treatment (P < 0.05); Before and after treatment for one month, the concentration of EGF expression in GCF was significantly higher than blood, saliva (P < 0.05); After treatment, the GI, PBI, PD levels were significantly lower than before treatment (P < 0.05); After treatment, flow of saliva and GCF were significantly higher than before treatment (P < 0.05).Conclusion Orthodontic treatment has certain influence on EGF expression in GCF, but has no obvious influence on EGF expression in blood and saliva; The increase of EGF expression derives from periodontal tissue cells and has close association with periodontal remodeling, periodontal tissue cells can promote self-remodeling through autogenous regulation and local humoral regulation.
Objective To investigate the changes of α2-macroglobulin in different stages of radiotherapy in patients with nasopharyngeal carcinoma, and to explore its feasibility as a marker of serum markers reflecting radiotherapy injury.Methods We collected the blood samples of 23 cases of newly diagnosed patients with nasopharyngeal carcinoma before the simple radiotherapy, the 10, 20, 30 and 33 times after simple radiotherapy, detected the α2- macroglobulin levels. The difference among the five stages was analysed by paired t-test using SPSS17.0 software package.Results The serum level of α2- macroglobulin elevated with the increase of number of radiotherapy. After 10 times’ radiotherapy, the serum α2-MG concentration in patients with nasopharyngeal carcinoma was significantly higher than that before radiotherapy (12.04 ± 5.72 vs. 10.81 ± 5.38 U/L), the difference was statistically significant (t=4.818, P < 0.05). After 20 times’ radiotherapy, the serum α2-MG concentration in patients with nasopharyngeal carcinoma was significantly higher than that before radiotherapy (12.26 ± 5.77 vs. 10.81 ± 5.38 U/L), and the difference was statistically significant (t=5.237, P < 0.001). After 30 times’ radiotherapy, the serum α2-MG concentration in patients with nasopharyngeal carcinoma was significantly higher than that before radiotherapy (12.91 ± 5.55 vs. 10.81 ± 5.38 U/L), the difference was statistically significant (t=6.076, P < 0.05). At the end of radiotherapy, the serum α2-MG concentration in nasopharyngeal carcinoma patients was significantly (13.43 ± 6.05 vs. 10.81 ± 5.38 U/L) higher than that before radiotherapy (t=5.189, P < 0.05).Conclusion The serum level of α2- macroglobulin changes with the radiotherapy, so it can be a serum marker reflecting the damage of maxilla induced by ionizing radiation.
Objective To investigate the clinical effect of dissection in the upper part of parotid gland benign tumor using a tragus edge combined retroauticular hairline incision approach.Methods 20 cases of upper part of parotid gland benign tumor were resected using the tragus edge combined retroauticular hairline incision approach, the surgery and cosmetic effect were analyzed. Results Parotid gland tumors were checked by CT before the operation and diagnosed by frozen section analysis during the operation. The tumors were successfully complete resected in all cases. There were no complications of Frey’s syndrome, postoperative bleeding, and flap necrosis, 2 cases showed a temporary earlobe numbness, 1 case of temporary facial nerve paralysis and 1 case of salivary fistula. There were no tumor recurrence after 24 ~ 48 months followed up. Surgical incision cosmetic effect is satisfactory. Conclusion The tragus edge combined retroauticular hairline incision is safe and feasible, with less complications for the upper part of parotid gland benign tumor resecetion.
Objective To investigate the knowledge of pupils’ parents for the emergency treatment of dental trauma in Chancheng of Foshan. Methods By the method of random sampling, a total of 320 parents from 5 primary schools of Chancheng District was selected for questionnaire survey on the treatment method of children’s dental trauma. Chi square test was used for statistical analysis.Results 40.6% of the parents didn’t know that the anterior teeth of a nine-year-old child was permanent. 66.2% of parents didn’t know suitable methods to deal with the avulsed tooth. 72.2% parents didn’t selected the corrected preservation medium for the avulsed tooth. 95.6% of parents believed they needed to learn more knowledge of emergency treatment for dental trauma. Conclusion The present study showed that the knowledge of parents in Chancheng of Foshan about the emergency treatment of dental trauma is inadequate and related education is necessary.
In the process of children’s dental treatment, children’s non-cooperation has always troubled dentists in clinical work. Using behavior management techniques properly can help us manage children’s behavior more effectively, improve the treatment efficiency, and greatly relieve the work stress. This paper reviewed the research progress on the basic and advanced behavior techniques for children’s oral behavior management techniques.
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