Journal of Prevention and Treatment for Stomatological Diseases ›› 2017, Vol. 25 ›› Issue (4): 223-226.doi: 10.12016/j.issn.2096-1456.2017.04.004

• Basic Study • Previous Articles     Next Articles

Effects of arecoline on apoptosis of human periodontal ligament fibroblasts

Huan-ling WANG1(), Jian-ping ZHANG2()   

  1. 1. Lushan Mountain Road Community Health Service Center of Tianjin, Tianjin 300171, China
    2. Second Affiliated Hospital of Tianjin University of TCM, Tianjin 300171, China
  • Received:2016-08-26 Revised:2016-11-08 Online:2017-04-20 Published:2018-09-03

Abstract:

Objective To observe the effects of arecoline on the related protein expressions in the apoptosis of cultured human periodontal ligament fibroblasts (hPDLFs), such as p-JNK, p-p53, and Bcl-2. Methods hPDLFs were isolated from human periodontal ligament tissues and expanded in vitro. hPDLFs were treated with different concentrations of arecoline (0, 20, 40 and 80 μg/mL) for 12 h. The proliferative activities were evaluated by MTT. The expressions of p-JNK, p-p53, and Bcl-2 were determined by Western blot. Results Arecoline inhibited cell proliferation and induced apoptosis protein. The protein level of Bcl-2 was decreased, while p-JNK and p-p53 were increased (P < 0.05). The protein expressions were in a concentration-dependent manner with arecoline. Conclusion It demonstrates arecoline might contribute to the apoptosis of hPDLFs, and could destroy periodontal tissues.

Key words: Arecoline, Human periodontal ligament fibroblasts, Apoptosis, Inhibition, Protein expression

CLC Number: 

  • R781.4

Figure 1

hPDLFs and immunofluorescence identification"

Table 2

Proliferation activity of hPDLFs and expressions of p-JNK, p-p53 and Bcl-2 protein(n = 3)"

组别(μg/mL) OD值(A) P p-JNK P p-p53 P Bcl-2 P
0(对照组) 0.88 ± 0.08 1.00 ± 0.00 1.00 ± 0.00 1.00 ± 0.00
20 0.76 ± 0.06 0.290 0.92 ± 0.14 0.175 1.45 ± 0.08 0.093 0.88 ± 0.06 0.059
40 0.68 ± 0.12 0.039 1.48 ± 0.01 0.025 1.72 ± 0.04 0.033 0.42 ± 0.05 0.028
80 0.47 ± 0.11 0.021 1.97 ± 0.12 0.022 2.28 ± 0.13 0.004 0.18 ± 0.03 0.017

Figure 2

Flow cytometry analysis of apoptosis of hPDLFs induced by arecoline"

Figure 3

Expressions of p-JNK, p-p53 and Bcl-2 in PDLFs induced by arecoline"

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