Journal of Prevention and Treatment for Stomatological Diseases ›› 2017, Vol. 25 ›› Issue (5): 294-299.doi: 10.12016/j.issn.2096-1456.2017.05.004

• Basic Study • Previous Articles     Next Articles

Expression of autophagy-related proteins PTEN and MAP1LC3 in tongue squamous cell carcinoma

Ya-nan WANG1(), Hao LIU2(), Jun ZHANG2   

  1. 1. Tianjin Medical University, Tianjin 300000, China
    2. Oral and Maxillofacial Surgery of Tianjin Stomatological Hospital of NanKai University, Tianjin 300000, China
  • Received:2016-11-13 Revised:2016-12-23 Online:2017-05-20 Published:2018-09-03

Abstract:

Objective This study ai ms to evaluate the expression of autophagy-related protein cancer phosphatase and tensinhomology deleted on chromosome ten (PTEN) and microtubule-associated protein 1 light chain 3 (MAP1LC3) and investigate its significance in tongue squamous cell carcin oma (TSCC). Methods About 46 TSCC samples, 13 cases of precancerous lesion and 13 cases of adjacent tissues were enrolled. Immunohistochemistry were performed to examine the expression of PTEN, MAP1LC3. Statistical analyses were carried out to assess the associations among clinic pathologic parameters. Results The positive expression rate of PTEN (34.78%) in TSCC was significantly lower than that in adjacent tissues (69.23%, χ2 = 4.926, P = 0.026) and precancerous lesions (76.92%, χ2 = 7.302, P = 0.007). The positive expression rate of MAP1LC3 (28.26%) in TSCC was significantly lower than that of adjacent tissues (61.53%, χ2 = 4.896, P = 0.027) and precancerous lesions (69.23%, χ2 = 7.275, P = 0.007), the difference was statistically significant. The expression of PTEN and MAP1LC3 was not correlated with age, sex, smoking, alcohol consumption and cell differentiation (P > 0.05). There was a significant difference in the expression of PTEN and MAP1LC3 in subgroup with different clinical stage and in subgroup with or without lymph node metastasis (P < 0.05). There is a significant difference in the tumor recurrence between PTEN protein expression positive subgroup and PTEN protein expression negative subgroup (χ2 = 4.629,P = 0.039), and there was no significant difference in the tumor recurrence between MAP1LC3 protein expression positive subgroup and MAP1LC3 protein expression negative subgroup (χ2 = 0.343,P = 0.453). There was a positive correlation between PTEN and MAP1LC3. Conclusion The expression of PTEN and MAP1LC3 suggested that autophagy associated proteins play a pivotal role in the progression, diagnosis and prognosis of TSCC. Down-regulation of PTEN and MAP1LC3 was observed in TSCC.

Key words: Tongue squamous cell carcinoma, Autophagy, PTEN, MAP1LC3, Prognosis

CLC Number: 

  • R739.8

Figure 1

The expression of MAP1LC3 and PTEN on tongue cell squamous cell carcinomas, oral precancer lesions and adjacent tissues IHC × 200"

Table 2

Association of PTEN and MAP1LC3 expression with clinical pathologic variables in tongue squamous cell carcinoma n "

临床病理特征 例数 PTEN蛋白表达 MAP1LC3蛋白表达
阳性 阴性 χ2 P 阳性 阴性 χ2 P
性别
30 13 17 2.780 0.095 8 22 0.108 0.742
16 3 13 5 11
年龄
≤60 26 8 18 0.425 0.515 5 21 2.405 0.121
>60 20 8 12 8 12
吸烟
25 8 17 0.187 0.665 7 18 0.002 0.966
21 8 13 6 15
饮酒
18 7 11 0.220 0.639 6 12
28 9 19 7 21 0.375 0.540
临床分期
Ⅰ期 10 7 3 10.645 0.0101) 5 5
Ⅱ期 13 6 7 5 8 5.788 0.122
Ⅲ期 11 2 9 1 10
Ⅳ期 12 1 11 2 10
临床分期
Ⅰ+Ⅱ期 23 13 10 9.583 0.0051) 10 13 5.254 0.0221)
Ⅲ+Ⅳ期 23 3 20 3 20
病理分级
高分化 25 13 12 3.450 0.063 8 17 0.985 0.326
中/低分化 21 3 18 5 16
淋巴结转移
17 2 15 7.362 0.0251) 1 16 5.024 0.0411)
29 14 15 12 17

Table 3

Relationship between tumor recurrence and PTEN and MAP1LC3 expression n"

复发 例数 PTEN蛋白表达 MAP1LC3蛋白表达
阳性 阴性
χ2
P值 阳性 阴性 χ2 P
7 1 6 4.269 0.039 2 5 0.343 0.453
22 13 9 9 13
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