Journal of Prevention and Treatment for Stomatological Diseases ›› 2021, Vol. 29 ›› Issue (8): 523-528.DOI: 10.12016/j.issn.2096-1456.2021.08.003

• Basic Study • Previous Articles     Next Articles

In vitro study of different methods in the removal of calcium hydroxide from root canals

TANG Lan(),PENG Bin()   

  1. Key Laboratory for Oral Biomedical Engineering of Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan 430079, China
  • Received:2021-01-27 Revised:2021-02-20 Online:2021-08-20 Published:2021-05-13
  • Contact: Bin PENG
  • Supported by:
    National Natural Science Foundation of China(81970935)



  1. 武汉大学口腔医学院口腔生物医学工程教育部重点实验室,湖北 武汉(430079)
  • 通讯作者: 彭彬
  • 作者简介:唐澜,硕士研究生,
  • 基金资助:


Objective To compare the efficacy of different methods in the removal of calcium hydroxide from root canals and to provide a reference for clinical treatment. Methods A total of 160 extracted single-rooted mandibular premolars were instrumented up to ProTaper Universal F4. The roots were split longitudinally, and standardized groove and depression models were prepared and filled with calcium hydroxide. The samples were randomly divided into 4 groups (n=40) according to different irrigation methods: syringe needle irrigation, passive ultrasonic irrigation, XP-endo finisher (XPF) irrigation, and M3-Max irrigation. Each group was then divided into 2 subgroups (n=20) according to the irrigation protocol: NaOCl and NaOCl+EDTA. Photos of grooves and depressions were taken under a microscope after irrigation, and the residual calcium hydroxide was scored to compare the removal effects of different irritation methods and solutions. Results In the groove and depression model, when sodium hypochlorite is used as the irrigation fluid, ultrasound irrigation, XPF and M3-Max are better than syringe needle irrigation in removing calcium hydroxide (P < 0.05); when sodium hypochlorite combined with EDTA flushing, the effect of removing calcium hydroxide with ultrasound irrigation, XPF and M3-Max is better than that of syringe needle irrigation (P < 0.05); but there is no statistically significant difference between ultrasound, XPF and M3-Max (P > 0.05); when compared with the use of sodium hypochlorite, the combined use of EDTA irrigation could enhance the effect of ultrasonic irrigation, XPF and M3 Max on the removal of calcium hydroxide (P < 0.05), but there was no significant improvement in the syringe needle irrigation group (P > 0.05). Conclusion Sodium hypochlorite combined with EDTA can enhance the effect of ultrasonic irrigation, XPF and M3 Max on the removal of calcium hydroxide, and there is no significant difference among these approaches, which are more effective than syringe needle irrigation.

Key words: ultrasonic irrigation, syringe needle irrigation, XP-endo finisher, M3-Max, calcium hydroxide, NaClO, ethylene diamine tetraacetic acid


目的 比较不同冲洗方法对根管内氢氧化钙的清除效果,为临床治疗提供参考。方法 收集160颗下颌单根管恒前磨牙,截冠后ProTaper预备至F4。将牙纵向劈为两半,分别在根管壁上制备标准化凹槽和凹陷模型并填入氢氧化钙。根据不同冲洗方法将样本随机分为4组(n=40):注射器组、超声组、XP-endo finisher(XPF)组、M3-Max组;每组根据冲洗液再分为2个亚组(n=20):次氯酸钠组、次氯酸钠+乙二胺四乙酸(ethylene diamine tetraacetic acid,EDTA)组。冲洗结束后显微镜下拍摄凹槽和凹陷照片,并对残余氢氧化钙进行评分,比较不同冲洗方法和冲洗液对氢氧化钙的清除效果。结果 在凹槽和凹陷模型中,使用次氯酸钠作为冲洗液时,超声、XPF和M3-Max清除氢氧化钙的效果均优于注射器冲洗(P < 0.05);当使用次氯酸钠联合EDTA冲洗时,超声、XPF和M3-Max清除氢氧化钙的效果也均优于注射器冲洗(P < 0.05);但超声、XPF和M3-Max三者之间的差异均无统计学意义(P > 0.05);与使用次氯酸钠作为冲洗液时相比,次氯酸钠联用EDTA冲洗可增强超声、XPF和M3-Max清除氢氧化钙的效果(P < 0.05),但不能增强注射器冲洗效果(P > 0.05)。结论 次氯酸钠联用EDTA冲洗可增强超声、XPF和M3-Max清除氢氧化钙的效果,三者间无明显差异,均优于注射器冲洗。

关键词: 超声冲洗, 注射器冲洗, XP-endo finisher, M3-Max, 氢氧化钙, 次氯酸钠, 乙二胺四乙酸

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