Journal of Prevention and Treatment for Stomatological Diseases ›› 2021, Vol. 29 ›› Issue (8): 535-540.DOI: 10.12016/j.issn.2096-1456.2021.08.005

• Basic Study • Previous Articles     Next Articles

The effect of silencing the endoplasmic reticulum stress-related protein calnexin on the proliferation, invasion, and migration of tongue squamous cell carcinoma cells

ZHONG qijian1(),JIN Tingting2,PENG Yu3,CHEN Weixiong2(),LI Jinsong3()   

  1. 1. Department of Stomatology, University of Chinese Academy Sciences-Shenzhen Hospital, Shenzhen 518106, China
    2. Department of Stomatology, Longgang District Central Hospital, Affliated to Guangzhou University of Traditional Chinese Medicine, Shenzhen 518116, China
    3. Department of Oral and Maxillofacial Surgery, Sun Yat-Sen Memorial Hospital of Sun Yat-Sen University, Guangzhou 510120, China
  • Received:2021-01-25 Revised:2021-03-01 Online:2021-08-20 Published:2021-05-13
  • Contact: Weixiong CHEN,Jinsong LI
  • Supported by:
    National Natural Science Foundation of China(82002861);Guangdong Basic and Applied Basic Research Foundation(2019A1515110124);China Postdoctoral Science Foundation(2019M663114)

沉默calnexin对舌鳞状细胞癌细胞增殖、侵袭和迁移的影响

钟齐健1(),金婷婷2,彭毓3,陈伟雄2(),李劲松3()   

  1. 1.中国科学院大学深圳医院(光明)口腔科,广东 深圳(518106)
    2.广州中医药大学附属深圳市龙岗中心医院口腔科,广东 深圳(518116)
    3.中山大学孙逸仙纪念医院口腔颌面外科,广东 广州(510120)
  • 通讯作者: 陈伟雄,李劲松
  • 作者简介:钟齐健,主治医师,硕士,Email:546276915@qq.com
  • 基金资助:
    国家自然科学基金项目(82002861);广东省基础与应用基础研究基金(2019A1515110124);中国博士后科学基金资助项目(2019M663114)

Abstract:

Objective To investigate the effect of silencing the endoplasmic reticulum stress-related protein calnexin on the proliferation, invasion, and migration of tongue squamous cell carcinoma cells. Methods Calnexin siRNA was transfected into SCC-9 and SCC-25 tongue squamous cell carcinoma cells, and the expression of calnexin was detected by qRT-PCR. The silencing effect of calnexin siRNA was further verified by Western blotting. CCK-8 assay was applied to detect the effect of silencing calnexin on the proliferation of tongue squamous cell carcinoma cells; Transwell assay was used to detect the effect of silencing calnexin on the invasion and migration of tongue squamous cell carcinoma cells. Results qRT-PCR showed that calnexin siRNA could effectively downregulate the expression of calnexin. Western blot analysis further confirmed the silencing effect of calnexin siRNA on calnexin. The CCK-8 assay showed that silencing calnexin expression on the 4th and 5th days could inhibit the proliferation of tongue squamous cell carcinoma cells, and the difference was statistically significant (P < 0.01). The Transwell assay showed that knockdown of calnexin could inhibit the invasion and migration of tongue squamous cell carcinoma cells (P < 0.001). Conclusion Knockdown of calnexin can inhibit the proliferation, invasion, and migration of tongue squamous cell carcinoma cells.

Key words: endoplasmic reticulum stress, calnexin, tongue squamous cell carcinoma, SCC-9 cells, SCC-25 cells, proliferation, invasion, migration, siRNA, therapeutic target

摘要:

目的 探讨沉默内质网应激相关蛋白calnexin对舌鳞状细胞癌细胞增殖、侵袭和迁移的影响。方法 将calnexin siRNA转染到舌鳞状细胞癌细胞SCC-9 和SCC-25中,采用qRT-PCR检测calnexin表达情况;Western blot实验进一步验证calnexin siRNA沉默效果。通过CCK-8实验检测沉默calnexin对舌鳞状细胞癌细胞增殖的影响;运用Transwell法检测沉默calnexin对细胞侵袭和迁移能力的影响。结果 qRT-PCR显示calnexin siRNA能够有效沉默calnexin的表达;Western blot实验进一步证实calnexin siRNA对calnexin的沉默效果。CCK-8实验显示沉默calnexin表达的第4天,第5天能够抑制舌鳞状细胞癌细胞的增殖,差异有统计学意义(P < 0.01);Transwell实验提示沉默calnexin的表达能够抑制舌鳞状细胞癌细胞的侵袭和迁移(P < 0.001)。结论 沉默calnexin可抑制舌鳞状细胞癌细胞的增殖、侵袭和迁移。

关键词: 内质网应激, calnexin, 舌鳞状细胞癌, SCC-9细胞株, SCC-25细胞株, 增殖, 侵袭, 迁移, siRNA, 治疗靶点

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