Journal of Prevention and Treatment for Stomatological Diseases ›› 2017, Vol. 25 ›› Issue (1): 21-25.doi: 10.12016/j.issn.2096-1456.2017.01.004

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Effects of arecoline and calcium ion on the permeability of dysplastic oral epithelia model in vitro

Dong-lan HOU1,Rong CHEN1,Yi-jun GAO1,Xiao-min YIN2()   

  1. 1. Oral Medical Center of the Second Xiangya Hospital of Central South University, Changsha 410011, China
    2. Center of Stomatology of Xiangya hospital, Central South University, Changsha 410008, China
  • Received:2016-05-29 Revised:2016-08-12 Online:2017-01-20 Published:2017-01-20
  • Contact: Xiao-min YIN E-mail:yxm6569@163.com

Abstract:

Objective To study the effects of arecolineand Calcium ion (Ca 2+) on the permeability of dysplastic oral epithelia model in vitro. Methods To establish the dysplastic oral epithelia model in vitro by culturing oral keratinocyte(DOK) and harvesting a DOK cell monolayer. The models were divided into control group, arecoline group and "Ca 2++ arecoline" group. The values of Lucifer Yellow Papp were used to estimate the permeability changes of models after treatedwith arecoline and Ca 2+. Results In arecoline group, the lucifer yellow Papp values of each subgroup were higher than that of control group (P < 0.05), and the values increased as arecoline concentration elevated and time lasted (P < 0.05). When the "Ca 2++ arecoline" group were pretreated with Ca 2+, the values of subgroup "10 μg/mL" was lower than that of the corresponding arecoline group (P > 0.05). However, the value of subgroup "4 h 10 μg/mL" of "Ca 2++ arecoline" group had no statistical difference with that of control group (P > 0.05), while the other subgroups were increased (P < 0.05); Besides, these values in "Ca 2++ arecoline" group were increased as the arecoline concentration elevated and time lasted too (P < 0.05). Conclusion Intervention of arecoline contributes to the increase of permeability of DOK cell monolayer model, which maybe an important reason for the cancerization of dysplastic oral epithelia, however Ca 2+might weaken these effects of arecoline in the process.

Key words: Arecoline, Dysplastic oral epithelia, In vitro model, Calcium, Permeability

CLC Number: 

  • R781.5

Figure 1

Morphology of DOK by inverted microscope × 200"

Table 1

Papp of lucifer yellow of different DOK cell monolayer models after treated with arecoline or Ca2++ arecoline"

组别 4 h 8 h 12 h
对照组(A组) 2.27±0.66 2.23±0.33 2.29±0.56
槟榔碱处理组(B组) 10 μg/mL 2.39±0.63 2.56±0.21 2.81±0.30
20 μg/mL 2.59±0.31 2.79±0.33 3.12±0.43
30 μg/mL 2.77±0.59 3.03±0.18 3.40±0.68
钙离子+槟榔碱处理组
(C组)
10 μg/mL 2.23±0.40 2.37±0.56 2.59±0.36
20 μg/mL 2.35±0.38 2.56±0.27 2.85±0.19
30 μg/mL 2.51±0.21 2.79±0.41 3.16±0.60
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