Journal of Prevention and Treatment for Stomatological Diseases ›› 2017, Vol. 25 ›› Issue (6): 360-364.doi: 10.12016/j.issn.2096-1456.2017.06.004

• Basic Study • Previous Articles     Next Articles

Effects of non-surgical periodontal treatment on level of vaspin and TNF-α in gingival crevicular fluid of type 2 diabetic patients with chronic periodontitis

Lihong WANG1(), Wenbiao ZHANG1, Lijun XI1, Aiqin BAO1, Shiguang HUANG2()   

  1. 1. Department of Stomotology Shenzhen Futian Hospital of Traditional Chinese Medicine Shenzhen 518034 China
    2. Faculty of Dentistry School of Medicine Jinan University Guangzhou 510632 China
  • Received:2017-01-04 Revised:2017-03-28 Online:2017-06-20 Published:2018-09-03

Abstract:

Objective To investigate the changes of vaspin and TNF-α levels in gingival crevicular fluid (GCF) of type 2 diabetic (T2DM) patients with chronic periodontitis (CP) after non-surgical periodontal treatment. Methods 60 subjects were divided into 4 groups: DM-CP group (patients with both T2DM and CP, n=15); CP group (CP patients without T2DM, n=15); DM group (T2DM patients without CP, n=15), and CTRL group (systemically and periodontally healthy individuals, n=15). The clinical parameters of periodontal tissue and GCF were measured before and 8 weeks after non-surgical periodontal treatment. Results The levels of vaspin and TNF-α were measured by ELISA. The levels of vaspin and TNF-α in CP group were significantly higher than those in CTRL group (P < 0.05), while the levels of vaspin and TNF-α in CP group were significantly decreased after treatment (P < 0.05). There was a statistically significant positive correlation between the total amount of vaspin and the total amount of TNF-α, the level of HbA1c, gingival index (GI) and probing depth (PD) (P < 0.05). Conclusion The results shows that vaspin and TNF-α are greatly decreased in periodontitis after non-surgical periodontal treatment. It suggests that vaspin and TNF-α in GCF may serve as inflammatory markers for the diagnosis and prognosis of diabetes and periodontitis.

Key words: Visceral adipose tissue-derived serine protease inhibitor, Tumor necrosis factor-alpha, Gingival crevicular fluid, Periodontitis, Type 2 diabetes mellitus

CLC Number: 

  • R781.4

Table 1

Basic information of subjects"

组别 性别
(男∶女)
年龄
(岁)
BMI
(kg/m2
HbAlc
(%)
糖尿病患病时间
(年)
DM-CP组 9∶6 49.52 ± 5.12 22.96 ± 1.20 7.43 ± 0.39* 3.70 ± 0.64
CP组 8∶7 48.75 ± 5.25 22.91 ± 1.51 5.00 ± 0.12
DM组 9∶6 48.27 ± 6.08 22.82 ± 1.89 7.39 ± 0.73* 3.73 ± 0.62
CTRL组 7∶8 47.50 ± 5.42 22.96 ± 1.20 5.00 ± 0.26

Table 2

Periodontal clinical indexes of full mouth and sampling sites before and after treatment (x?± s)"

组别
牙周临床指标
治疗前 治疗后
GI BOP(%) PD(mm) GI BOP(%) PD(mm)
DM-CP组(全口) 2.19 ± 0.11* 71.09 ± 0.97* 3.78 ± 0.25* 0.79 ± 0.07** 8.43 ± 0.40** 2.58 ± 0.08**
DM-CP组(取样位点) 2.26 ± 0.11* 100.00 ± 0.00* 5.84 ± 0.14* 0.31 ± 0.05** 0.35 ± 0.05** 2.55 ± 0.08**
CP组(全口) 2.14 ± 0.03* 70.92 ± 0.25* 3.99 ± 0.06* 0.82 ± 0.02** 8.77 ± 0.05** 2.54 ± 0.03**
CP组(取样位点) 2.17 ± 0.03* 100.00 ± 0.00* 5.99 ± 0.06* 0.28 ± 0.03** 0.38 ± 0.02** 2.50 ± 0.03**
DM组(全口) 0.21 ± 0.03 8.39 ± 0.08 2.59 ± 0.07 / / /
DM组(取样位点) 0.00 ± 0.00 0.00 ± 0.00 2.05 ± 0.03 / / /
CTRL组(全口) 0.17 ± 0.02 6.02 ± 0.08 2.38 ± 0.12 / / /
CTRL组(取样位点) 0.00 ± 0.00 0.00 ± 0.00 2.21 ± 0.09 / / /

Table 3

Total amount of vaspin in GCF before and after treatment (x?± s,ng)"

组别 DM-CP组 CP组 DM组 CTRL组
治疗前 0.91 ± 0.05* 0.82 ± 0.02* 0.38 ± 0.02 0.29 ± 0.02
治疗后 0.50 ± 0.03** 0.36 ± 0.01** / /

Table 4

Total amount of TNF-α in GCF before and after treatment (x ± s,pg)"

组别 DM-CP组 CP组 DM组 CTRL组
治疗前 8.95 ± 0.07* 7.69 ± 0.02* 2.67 ± 0.05 1.95 ± 0.06
治疗后 3.07 ± 0.02** 2.10 ± 0.02** / /

Table 5

Correlation between total amount of vaspin and total amount of TNF-α, the level of HbAlc and GI, PD values of sampling sites"

组别 相关性
分析
vaspin总量
与TNF-α
总量
vaspin
总量与
HbAlc水平
vaspin
总量与
GI值
vaspin
总量与
PD值
DM-CP组 r 0.923 0.940 0.935 0.914
P 0.000 0.000 0.000 0.000
CP组 r 0.751 0.757 0.882 0.642
P 0.001 0.001 0.000 0.010
DM组 r 0.951 0.957 / 0.913
P 0.000 0.000 / 0.000
CTRL组 r 0.863 0.882 / 0.807
P 0.000 0.000 / 0.000
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