Journal of Prevention and Treatment for Stomatological Diseases ›› 2017, Vol. 25 ›› Issue (8): 482-487.doi: 10.12016/j.issn.2096-1456.2017.08.002

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Changes of signal transducer and activator of transcription 1 expression in the anterior cingulate cortex under orthodontic force in rats

Yi ZHENG1, Zhuannong ZHAO2, Yixi WANG3, Yang CAO3(), Chufeng LIU2()   

  1. 1. Zhuhai Smile Dental Clinic Zhuhai 519000 China
    2. Department of Orthodontics Stomatological Hospital Southern Medical University Guangzhou 510280 China
    3. Department of Orthodontics Guanghua School of Stomatology Hospital of Stomatology Sun Yat-sen University Guangzhou 510055 China
  • Received:2017-04-10 Revised:2017-06-08 Online:2017-08-20 Published:2018-09-03

Abstract:

Objective To study the change of STAT1 expression in the anterior cingulate cortex on rats under orthodontic force, and to further explore the roles of STAT1 and related JAK-STAT1 signaling pathway in the mediation and regulation of pain during tooth movement. Methods 112 male Sprague-Dawley (SD) rats (225±25 g) were used in this study. They were randomly divided into experimental groups (96 rats) and control groups (16 rats). All rats were installed bilateral maxillary device for tooth movement models. Rats in the experimental groups were applied 80g orthodontic force on both sides and were divided into six subgroup 4 h, 12 h, 24 h, 2 d, 3 d, 7 d, with 16 rabbits in each subgroup. The control groups were only installed the same orthodontic devices, without the application of orthodontic force. Brain tissue of the anterior cingulate cortex was isolated after 4 h, 12 h, 24 h, 2 d, 3 d, 7 d since experiment, and the expression level of STAT1 and p-STAT1 was analyzed with the method of immunofluorescence and PCR. Results For the immunofluorescence result, there was significant difference in STAT1 expression between control groups and different experimental groups at different time points in total (P < 0.05). The STAT1 expression amount in the 4 h group decreased significantly when compared with the control group (P < 0.05); to the 2 d group, the difference is still statistically significant (P < 0.01). 3 d group, 7 d group and control group had no statistically significant difference. The STAT1 expression amount in 4 h group, 12 h group, 24 h group was significantly lower than that in 3 d and 7 d groups, differences were statistically significant (P < 0.05). The STAT1 expression in the 2 d group was significantly lower than that of 7 d (42.35 ± 5.77) group, the difference was statistically significant (P < 0.05). There was significant difference in p-STAT1 expression between control groups and different experimental groups at different time points in total (F = 623.518, P < 0.05). The p-STAT1 expression amount in experimental groups were higher than that in the control group (P < 0.05). The p-STAT1 expression in 4 h group was lower than that in 12 h and 24 h group and higher than that in 2 d, 3 d and 7 d groups, of which the differences were statistically significant (P < 0.05). The p-STAT1 expression in 12 h group was lower than that in 24 h group and higher than that in 2 d, 3 d and 7 d groups, of which the differences were statistically significant (P < 0.05). For the PCR result, the expression of mRNA in STAT1 of experimental groups of 4 h, 12 h, 24 h, 2 d, 3 d, 7 d and the control groups were not statistically significant (P > 0.05). Conclusions After applying orthodontic force, the expression of STAT1 decreased transiently and the expression of p-STAT1 increased transiently. The reduction of STAT1 was probably caused by the phosphorylation of STAT1 and decrease in the translation level of STAT1, rather than changes in the transcriptional levels. The orthodontic pain might be related with the activation of STAT1 into phosphorylated STAT1.

Key words: Orthodontic force, Pain, ACC, STAT1, pSTAT1

CLC Number: 

  • R783.5

Table 1

Primer sequences of STAT1 and β-actin"

引物 序列
STAT1-Forward 5′-CATGCCAACTCACCCTCAGA-3′
STAT1- Reverse
β-actin -Forward
β-actin -Reverse
5′-CAGCTGCCAAACTTCCGTTG-3′
5′-CATTGTCACCAACTGGGACGATA-3′
5′-GGATGGCTACGTACATGGCTG-3′

Figure 1

STAT1 expression in control and experimental group(Immunofluorescence × 200)"

Table 2

Comparison of STAT1 expression of different experimental groups(x?± s)"

组别 STAT1相对表达量 F P
对照组 42.23 ± 2.61 5.314 0.005
4 h组 25.21 ± 8.591)
12 h组 22.77 ± 5.131)
24 h组 22.33 ± 6.791)
2 d组 29.00 ± 10.251)
3 d组 38.33 ± 5.322)3)4)
7 d组 42.35 ± 5.772)3)4)5)

Figure 2

p-STAT1 expression in control and experimental group (Immunofluorescence × 200)"

Figure 3

Expression level of STAT1 mRNA in each group"

Table 3

Comparison of STAT1 expression of control group and different experimental groups (x?± s)"

组别 p-STAT1表达量 F P
对照组 10.67 ± 3.44 623.518 0.0007
4 h组 133.16 ± 5.711)
12 h组 175.27 ± 8.761)2)
24 h组 255.29 ± 6.591)2)3)
2 d组 73.97 ± 4.471)2)3)4)
3 d组 60.05 ± 5.531)2)3)4)5)
7 d组 32.68 ± 6.311)2)3)4)5)6)
[1] Rainville P, Duncan GH, Price DD, et al.Pain affect encoded in human anterior cingulate but not somatosensory cortex[J]. Science, 1997, 277(5328): 968-971.
[2] Ihle JN.Cytokine receptor signaling[J]. Nature, 1995, 377(6550): 591-594.
[3] Mori T, Miyamoto T, Yoshida H, et al.IL-1β and TNFα-initiated IL-6-STAT3 pathway is critical in mediating inflammatory cytokines and RANKL expression in inflammatory arthritis[J]. Int Immunol, 2011, 23(11): 701-712.
[4] Gracie JA, Robertson SE, Mc Innes IB.Interleukin-18[J]. J Leukoc Biol, 2003, 73(2):213-224.
[5] 陈宇, 辛隐子, 刘楚峰, 等. 不同正畸力值作用下大鼠脑前扣带回皮质层蛋白激酶Mξ的表达[J]. 中华口腔医学杂志, 2014, 49(12): 748-752.
[6] 赵转浓, 刘楚峰, 孙晓卫, 等. 前扣带回皮质层中牙移动疼痛相关蛋白质组学研究[J]. 中华口腔医学研究杂志(电子版), 2016, 10(2): 112-119.
[7] Kirschneck C, Proff P, Fanghaenel J, et al.Differentiated analysis of orthodontic tooth movement in rats with an improved rat model and three-dimensional imaging[J]. Ann Anat, 2013, 195(6): 539-553.
[8] Kiu H, Nicholson SE.Biology and significance of the JAK/STAT signalling pathways[J]. Growth Factors, 2012, 30(2): 88-106.
[9] Rane SG, Reddy EP.Janus kinases: components of multiple signaling pathways[J]. Oncogene, 2000, 19(49): 5662-5679.
[10] Stark GR, Kerr IM, Williams BR, et al.How cells respond to interferons[J]. Annu Rev Biochem, 1998, 67(25):227-264.
[11] Lödige I, Marg A, Wiesner B, et al.Nuclear export determines the cytokine sensitivity of STAT transcription factors[J]. J Biol Chem, 2005, 280(52): 43087-43099.
[12] Kose S, Imamoto N, Tachibana T, et al.Ran-unassisted nuclear migration of a 97-kD component of nuclear pore-targeting complex[J]. J Cell Biol, 1997, 139(4): 841-849.
[13] Melen K, Kinnunen L, Julkunen I.Arginine/lysine-rich structural element is involved in interferon-induced nuclear import of STATs[J]. J Biol Chem, 2001, 276(19): 16447-16455.
[14] Meyer T, Begitt A, Lödige I, et al.Constitutive and IFN-gamma-induced nuclear import of STAT1 proceed through Independent pathways[J]. EMBO J, 2002, 21(3): 344-354.
[15] Koritsánszky N Madléna M, Discomfort in Orthodontic Treatments. Literature review[J]. Fogorv Sz, 2011, 104(4): 117-121.
[16] Krishnan V.Orthodontic pain: from causes to management--a review[J]. Eur J Orthod, 2007, 29(2): 170-179.
[17] Patel S, Mcgorray SP, Yezierski R, et al.Effects of analgesics on orthodontic pain[J]. Am J Orthod Dentofacial Orthop, 2011, 139(1): e53-e58.
[18] Tortamano A, Lenzi DC, Haddad AC, et al.Low-level laser therapy for pain caused by placement of the first orthodontic archwire: A randomized clinical trial[J]. Am J Orthod Dentofacial Orthop, 2009, 136(5): 662-667.
[19] Wang J, Jian F, Chen J, et al.Cognitive behavioral therapy for orthodontic pain control: a randomized trial[J]. J Dent Res, 2012, 91(6): 580-585.
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