Journal of Prevention and Treatment for Stomatological Diseases ›› 2017, Vol. 25 ›› Issue (11): 687-692.doi: 10.12016/j.issn.2096-1456.2017.11.002

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Effect of Porphyromonas gingivalis lipopolysaccharide on gene expression of inflammatory cytokines in peritoneal macrophages of hyperlipidemic rabbits

Xiaoqing YOU1(), Kai LUO1, Yanfen LI1, Chunfang WU1, Fuhua YAN2()   

  1. 1. The affiliated Stomatological Hospital, Fujian Medical University, Key laboratory of Stomatology, Fujian Province University, Fujian Biological Materials Engineering and Technology Center of Stomatology, Fuzhou 350002, China
    2. Nanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing 210008, China
  • Received:2017-02-15 Revised:2017-04-23 Online:2017-11-20 Published:2018-09-03

Abstract:

Objective To compare the inflammatory reaction of peritoneal macrophage after Pg-LPS stimulated in healthy rabbit and hyperlipidemia rabbit. Methods 12 New Zealand rabbits were randomly divided into 2 groups with 6 rabbits in each group, and normal diet and high-fat diet were fed to them respectively. The hyperlipidemia model was set up after 6 weeks. The peritoneal macrophage in normal and hyperlipidemia group were isolated and cultured, and then the cells in both groups were respectively divided into 3 groups: control, 1 μg/mL Pg-LPS, and 1 μg/mL E.coli-LPS. After 24 h treatment, the expressions of C-reaction protein (CRP), interleukin-1β (IL-1β), interleukin-6 (IL-6) , interleukin-8 (IL-8), and tumor necrosis factor-α (TNF-α) were detected by realtime PCR. Results The levels of CRP, IL-1β, IL-6, IL-8, and TNF-α were higher in hyperlipidemia control group than normal control group. The expressions of inflammatory substances were increased after stimulated by Pg-LPS, and statistically higher in hyperlipidemia rabbit group than normal group (P < 0.05). Conclusion Pg-LPS can enhance the mRNA expressions of inflammation related factors in peritoneal macrophage in hyperlipidemia rabbit.

Key words: Porphyromonas gingivalis, Lipopolysaccharide, Hyperlipidemia, Peritoneal macrophage, Inflammation factor, Periodontitis

CLC Number: 

  • R781.4

Table 1

Lipid levels in normal and hyperlipidemia group mmol/L,x?±s"

组别 TC TG HDL-C LDL-C
高脂组(n = 6) 28.536 7 ± 3.708 02 3.758 3 ± 1.341 27 7.598 3 ± 2.125 46 37.320 0 ± 2.013 50
健康组(n = 6) 1.418 3 ± 0.457 23 0.696 7 ± 0.246 31 0.721 7 ± 0.305 97 0.521 7 ± 0.223 91
t 17.780 5.499 7.844 44.492
P < 0.001 0.002 < 0.001 < 0.001

Figure 1

Peritoneal macrophage in vitro 5 d Inverted phase contrast microscope × 100"

Figure 2

Peritoneal macrophage in vitro 12 d Inverted phase contrast microscope × 200"

Figure 3

Positive expression of MAC387 by immunocytochemical staining × 400"

Table 2

mRNA expressions of inflammation related factors after stimulated by Pg-LPS in peritoneal macrophage"

组别 CRP IL-1β IL-6 IL-8 TNF-α
健康兔 对照组 0.000 28 ± 0.000 17 0.002 18 ± 0.000 15 0.043 39 ± 0.001 45 0.125 76 ± 0.008 90 0.000 36 ± 0.000 01
E.coli-LPS组 0.000 50 ± 0.000 071) 0.029 48 ± 0.001 261) 0.065 23 ± 0.004 001) 0.267 03 ± 0.001 861) 0.002 28 ± 0.000 142)
Pg-LPS组 0.000 44 ± 0.000 021) 0.011 65 ± 0.001 411) 0.049 35 ± 0.000 77 0.135 03 ± 0.003 78 0.001 36 ± 0.000 112)
F 3.403 480.607 61.353 579.64 260.9811
P 0.103 < 0.001 < 0.001 < 0.001 < 0.001
高脂兔 对照组 0.010 04 ± 0.000 20 0.020 42 ± 0.000 40 0.149 67 ± 0.000 24 1.169 58 ± 0.000 39 0.001 27 ± 0.000 031)
E.coli-LPS组 0.014 37 ± 0.000 911) 0.055 68 ± 0.000 701) 0.388 47 ± 0.000 311) 3.209 93 ± 0.005 071) 0.005 68 ± 0.000 031)2)
Pg-LPS组 0.014 37 ± 0.001 981) 0.031 40 ± 0.000 201) 0.204 48 ± 0.000 191) 2.343 63 ± 0.003 161) 0.004 26 ± 0.000 161)2)
F 11.746 4 246.405 741 953.87 263 313.65 29.387 2
P 0.008 < 0.001 < 0.001 < 0.001 0.008

Figure 4

mRNA expressions of inflammation related factors after stimulated by Pg-LPS in peritoneal macrophage"

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