Journal of Prevention and Treatment for Stomatological Diseases ›› 2017, Vol. 25 ›› Issue (11): 712-717.doi: 10.12016/j.issn.2096-1456.2017.11.007

• Basic Study • Previous Articles     Next Articles

Angiogenesis-related factors expression in oral lichen planus

Wenxia MENG(), Wei GUO, Zhiqiang LI, Baoyi XIE, Cao YIN, Yanmei YAO, Juxiong LI   

  1. Stomatological Hospital, Southern Medical University, Guangzhou 510280, China
  • Received:2017-08-07 Revised:2017-09-19 Online:2017-11-20 Published:2018-09-03

Abstract:

Objective To detect the expression of vascular endothelial growth factor (VEGF), intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1) in oral lichen planus (OLP). Methods Thirty OLP patients and fifteen healthy subjects were enrolled in the study. Serum were collected from 15 healthy volunteers as controls. Normal tissues were collected from surgical department as immunohistochemical analysis. The levels of VEGF, ICAM-1, VCAM-1 in serum were measured by ELISA. Immunohistochemical analysis of VEGF, ICAM-1, VCAM-1 were carried out by the means of primary antibodies and anti-VEGF, anti-CD106 antigen (VCAM-1) and anti-CD54 antigen (ICAM-1). Results ELISA results showed no expression differences for VEGF between the two groups. Whereas, the levels of ICAM-1, VCAM-1 in OLP group were significantly higher than those in control group (P < 0.05). Immunohistochemical results reveal the presence of a significant angiogenesis in OLP patients through the immunoexpression of VEGF, ICAM-1, VCAM-1 according to the percentage of stained cells (P < 0.05). Conclusion Regarding the results, it seems that high expression of VCAM-1 and ICAM-1 are related to oral lichen planus.

Key words: Oral lichen planus, Angiogenesis, ELISA, VEGF, ICAM-1, VCAM-1

CLC Number: 

  • R781.5

Table 1

Total amount of VEGF、ICAM-1、VCAM-1 in Lichen planus and normal group $\bar{x}$± s,pg/mL"

组别 VEGF ICAM-1 VCAM-1
扁平苔藓组 0.145 ± 0.096 278.023 ± 60.133 728.395 ± 207.599
正常组 0.077 ± 0.044 229.127 ± 39.219 598.863 ± 135.687
P 0.074 0.009 0.035
t 1.837 2.730 2.167

Figure 1

The expression of VEGF on normal and oral lichen planus tissues IHC"

Figure 2

The expression of ICAM-1 on normal and oral lichen planus tissues IHC"

Figure 3

The expression of VCAM-1 on normal and oral lichen planus tissues IHC"

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